Cloned (Comment) | Organism |
---|---|
- |
Thermomyces lanuginosus |
Protein Variants | Comment | Organism |
---|---|---|
Q1C/Q24C | introduction of disulfide bridge, leading to increase in temperature optimum at pH 6.5 by about 10 degrees to 75°C, increased resistance to thermal inactivation and increased melting temperature. At pH 8 and 70°C, the disulfide bridge increases the enzyme half-life 20fold in the presence of substrate | Thermomyces lanuginosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermomyces lanuginosus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | O43097 | - |
- |
Thermomyces lanuginosus DSM 10635 | O43097 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oat xylan + H2O | - |
Thermomyces lanuginosus | ? | - |
? | |
oat xylan + H2O | - |
Thermomyces lanuginosus DSM 10635 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
XynA | - |
Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
wild-type | Thermomyces lanuginosus |
75 | - |
mutant Q1C/Q24C | Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
66 | - |
melting temperature, wild-type | Thermomyces lanuginosus |
70 | - |
wild-type at pH 8.0, half-life 11 min in presence of substrate, 33 min in absence of substrate, respectively. Mutant Q1C/Q24C at pH 8.0, half-life 229 min in presence of substrate, 315 min in absence of substrate, respectively. Mutant Q1C/Q24C at pH 9.0, half-life 112 min in presence of substrate, 169 min in absence of substrate, respectively | Thermomyces lanuginosus |
74 | - |
melting temperature, mutant Q1C/Q24C | Thermomyces lanuginosus |